Identification of N alpha-acetyl-alpha-endorphin and N alpha-acetyl-gamma-endorphin isolated from the neurointermediate lobe of the rat pituitary gland.
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چکیده
منابع مشابه
BIOSYNTHESIS AND RELEASE OF P-ENDORPHIN-, N-ACETYL p- ENDORPHIN-, /3-ENDORPHIN-(l-27)-, AND N-ACETYL P-ENDORPHIN- (l-27)-LIKE PEPTIDES BY RAT PITUITARY NEUROINTERMEDIATE LOBE: P-ENDORPHIN IS NOT FURTHER PROCESSED BY ANTERIOR LOBE’
Continuous labeling and pulse-chase techniques were employed to study the synthesis and secretion of multiple forms of immunoreactive ,f?-endorphin by cultured dispersed rat anterior lobe cells and intact neurointermediate pituitary lobe. Cell and medium extract immunoreactive pendorphin (specific immunoprecipitation and radioimmunoassay) exhibiting a K,, similar to authentic ,&endorphin upon g...
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An enzyme which N-acetylates beta-endorphin has been identified and characterized in the rat brain and pituitary gland. The beta-endorphin acetyltransferase activity was localized almost exclusively in the intermediate lobe of the pituitary gland. beta-endorphin acetyltransferase activity was also present in the hypothalamus, a brain region which synthesizes beta-endorphin, but not the cerebell...
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The proteolytic processing pattern of pro-ACTH/endorphin in rat hypothalamus is similar to the pattern in the pars intermedia; peptides the size of beta-endorphin, gamma-lipotropin (gamma-LPH), corticotropin-like intermediate lobe peptide (CLIP), alpha-melanotropin (gamma-MSH), joining peptide, and glycosylated gamma 3-MSH all represent predominant end products. Equimolar amounts of beta-endorp...
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This paper examines the distribution of fibers and cell bodies containing alpha-neo-endorphin in the cat brain stem by using an indirect immunoperoxidase technique. A high or moderate density of immunoreactive cell bodies was found in the superior central nucleus, nucleus incertus, dorsal tegmental nucleus, nucleus of the trapezoid body, and in the laminar spinal trigeminal nucleus, whereas a l...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)88831-7